Description
Aviva Systems Biology VKORC1 ELISA Kit (Bovine) (OKEH03847) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine VKORC1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated VKORC1 and incubated. The VKORC1 found in the sample or standards competes with the biotinylated VKORC1 for limited binding sites on the immobilized anti-bovine VKORC1 antibody. Excess unbound biotinylated VKORC1 and sample or standard VKORC1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated VKORC1 captured in the well and inversely proportional to the amount of VKORC1 which was contained in the sample or standard